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qubit 4 fluorometer  (Thermo Fisher)


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    Thermo Fisher qubit 4 fluorometer
    Qubit 4 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qubit+4+fluorometer/Agarose/bio_rxiv__64898__2026__04__04__716458-30-16-19
    Average 99 stars, based on 1 article reviews
    qubit 4 fluorometer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform
    Article Snippet: Amplicon pools were individually purified using Beckman Coulter AMPure XP beads (Beck-man Coulter, Indianapolis, IN, USA) following the manufacturer’s protocol. .. Following the PCR clean-up with AMPure beads, the DNA concentration for each amplicon pool was quantified using the Invitrogen Qubit 4 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) with the broad range dsDNA quantification kit. ..

    Article Title: Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform
    Article Snippet: Amplicons were individually purified using Beckman Coulter AMPure XP beads (Beck-man Coulter, Indianapolis, IN, USA) following the manufacturer’s protocol. .. Following the PCR clean-up with AMPure beads, the DNA concentration for each amplicon was quantified using the Invitrogen Qubit 4 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) with the broad range dsDNA quantification kit. ..

    Article Title: Super-pangenome analysis of 3562 human and animal papillomavirus isolates illuminates their genome and pathogenicity evolution
    Article Snippet: Final extension was set at 72 D for 5 min. For PCR product clean-up, we used the 1× VAHTS DNA Clean Beads (Cat: N411, Vazyme, China). .. The obtained HPV16 amplicons, containing three purified PCR amplicon fragments which were examined by electrophoresis and further quantified by Qubit 4 Fluorometer (Thermo Fisher, USA). .. For each sample, we mixed the three PCR amplicon fragments with a 1:1:1 mass ratio and sent for sequencing by BGI Genomics (China) using the BGI MGISEQ-2000 platform.

    Concentration Assay:

    Article Title: Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform
    Article Snippet: Amplicon pools were individually purified using Beckman Coulter AMPure XP beads (Beck-man Coulter, Indianapolis, IN, USA) following the manufacturer’s protocol. .. Following the PCR clean-up with AMPure beads, the DNA concentration for each amplicon pool was quantified using the Invitrogen Qubit 4 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) with the broad range dsDNA quantification kit. ..

    Article Title: Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform
    Article Snippet: Amplicons were individually purified using Beckman Coulter AMPure XP beads (Beck-man Coulter, Indianapolis, IN, USA) following the manufacturer’s protocol. .. Following the PCR clean-up with AMPure beads, the DNA concentration for each amplicon was quantified using the Invitrogen Qubit 4 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) with the broad range dsDNA quantification kit. ..

    Article Title: Whole genome sequence data of Comamonas sediminis FS4_11, a fumonisin B1-transforming bacterium, using hybrid nanopore-illumina sequencing
    Article Snippet: Genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit (Promega) following the manufacturer’s protocol. .. The genomic DNA quality was assessed using a NanoDrop 2000 (Thermo Fisher Scientific) for purity, a Qubit 4 Fluorometer (Thermo Fisher Scientific) for concentration, and agarose gel electrophoresis for integrity. .. Genome sequencing was outsourced to Biomarker Technologies (Beijing, China) using a hybrid approach combining long-read (Oxford Nanopore Technologies, ONT) and short-read (Illumina) sequencing.

    Article Title: A pseudo-phased genome assembly for Hemileia vastatrix reveals an isolate-specific chromosomal haploid trisomy
    Article Snippet: DNA samples were further purified using Pacific Biosciences (PacBio) SampleNet – Shared Protocol ( https://www.pacb.com/wp-content/uploads/2015/09/SharedProtocol-Extracting-DNA-usinig-Phenol-Chloroform.pdf ). .. DNA concentration and purity were measured using a Multiskan SkyHigh Microplate Spectrophotometer (Thermofisher Scientific) and a Qubit 4 Fluorometer (Thermofisher Scientific), and its quality checked on an agarose gel. .. Ten micrograms of high-quality HMW DNA were sent to CD Genomics (New York, USA) for SMRT bell size select long read library preparation and PacBio Sequel II sequencing.

    Amplification:

    Article Title: Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform
    Article Snippet: Amplicon pools were individually purified using Beckman Coulter AMPure XP beads (Beck-man Coulter, Indianapolis, IN, USA) following the manufacturer’s protocol. .. Following the PCR clean-up with AMPure beads, the DNA concentration for each amplicon pool was quantified using the Invitrogen Qubit 4 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) with the broad range dsDNA quantification kit. ..

    Article Title: Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform
    Article Snippet: Amplicons were individually purified using Beckman Coulter AMPure XP beads (Beck-man Coulter, Indianapolis, IN, USA) following the manufacturer’s protocol. .. Following the PCR clean-up with AMPure beads, the DNA concentration for each amplicon was quantified using the Invitrogen Qubit 4 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) with the broad range dsDNA quantification kit. ..

    Article Title: Super-pangenome analysis of 3562 human and animal papillomavirus isolates illuminates their genome and pathogenicity evolution
    Article Snippet: Final extension was set at 72 D for 5 min. For PCR product clean-up, we used the 1× VAHTS DNA Clean Beads (Cat: N411, Vazyme, China). .. The obtained HPV16 amplicons, containing three purified PCR amplicon fragments which were examined by electrophoresis and further quantified by Qubit 4 Fluorometer (Thermo Fisher, USA). .. For each sample, we mixed the three PCR amplicon fragments with a 1:1:1 mass ratio and sent for sequencing by BGI Genomics (China) using the BGI MGISEQ-2000 platform.

    Agarose Gel Electrophoresis:

    Article Title: Whole genome sequence data of Comamonas sediminis FS4_11, a fumonisin B1-transforming bacterium, using hybrid nanopore-illumina sequencing
    Article Snippet: Genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit (Promega) following the manufacturer’s protocol. .. The genomic DNA quality was assessed using a NanoDrop 2000 (Thermo Fisher Scientific) for purity, a Qubit 4 Fluorometer (Thermo Fisher Scientific) for concentration, and agarose gel electrophoresis for integrity. .. Genome sequencing was outsourced to Biomarker Technologies (Beijing, China) using a hybrid approach combining long-read (Oxford Nanopore Technologies, ONT) and short-read (Illumina) sequencing.

    Article Title: A pseudo-phased genome assembly for Hemileia vastatrix reveals an isolate-specific chromosomal haploid trisomy
    Article Snippet: DNA samples were further purified using Pacific Biosciences (PacBio) SampleNet – Shared Protocol ( https://www.pacb.com/wp-content/uploads/2015/09/SharedProtocol-Extracting-DNA-usinig-Phenol-Chloroform.pdf ). .. DNA concentration and purity were measured using a Multiskan SkyHigh Microplate Spectrophotometer (Thermofisher Scientific) and a Qubit 4 Fluorometer (Thermofisher Scientific), and its quality checked on an agarose gel. .. Ten micrograms of high-quality HMW DNA were sent to CD Genomics (New York, USA) for SMRT bell size select long read library preparation and PacBio Sequel II sequencing.

    Spectrophotometry:

    Article Title: A pseudo-phased genome assembly for Hemileia vastatrix reveals an isolate-specific chromosomal haploid trisomy
    Article Snippet: DNA samples were further purified using Pacific Biosciences (PacBio) SampleNet – Shared Protocol ( https://www.pacb.com/wp-content/uploads/2015/09/SharedProtocol-Extracting-DNA-usinig-Phenol-Chloroform.pdf ). .. DNA concentration and purity were measured using a Multiskan SkyHigh Microplate Spectrophotometer (Thermofisher Scientific) and a Qubit 4 Fluorometer (Thermofisher Scientific), and its quality checked on an agarose gel. .. Ten micrograms of high-quality HMW DNA were sent to CD Genomics (New York, USA) for SMRT bell size select long read library preparation and PacBio Sequel II sequencing.

    Synthesized:

    Article Title: N -acetylchitohexaose induces immune priming against Pseudomonas aeruginosa infection in the silkworm, Bombyx mori
    Article Snippet: RNA pellets were washed with 75% ethanol, air-dried, and dissolved in nuclease-free water (Qiagen, Hilden, Germany). .. RNA concentrations were measured using a Qubit 4 Fluorometer (Thermo Fisher Scientific). cDNA was synthesized using a commercial reverse transcription kit (ReverTra Ace, Toyobo, Osaka, Japan). .. Quantitative real-time PCR (qRT-PCR) was performed using Thunderbird Next SYBR qPCR Mix (Toyobo, QPX-201) on a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), with MicroAmp Fast 96-well Reaction Plates (Cat# 4346907, Applied Biosystems).

    Reverse Transcription:

    Article Title: N -acetylchitohexaose induces immune priming against Pseudomonas aeruginosa infection in the silkworm, Bombyx mori
    Article Snippet: RNA pellets were washed with 75% ethanol, air-dried, and dissolved in nuclease-free water (Qiagen, Hilden, Germany). .. RNA concentrations were measured using a Qubit 4 Fluorometer (Thermo Fisher Scientific). cDNA was synthesized using a commercial reverse transcription kit (ReverTra Ace, Toyobo, Osaka, Japan). .. Quantitative real-time PCR (qRT-PCR) was performed using Thunderbird Next SYBR qPCR Mix (Toyobo, QPX-201) on a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), with MicroAmp Fast 96-well Reaction Plates (Cat# 4346907, Applied Biosystems).

    Purification:

    Article Title: Super-pangenome analysis of 3562 human and animal papillomavirus isolates illuminates their genome and pathogenicity evolution
    Article Snippet: Final extension was set at 72 D for 5 min. For PCR product clean-up, we used the 1× VAHTS DNA Clean Beads (Cat: N411, Vazyme, China). .. The obtained HPV16 amplicons, containing three purified PCR amplicon fragments which were examined by electrophoresis and further quantified by Qubit 4 Fluorometer (Thermo Fisher, USA). .. For each sample, we mixed the three PCR amplicon fragments with a 1:1:1 mass ratio and sent for sequencing by BGI Genomics (China) using the BGI MGISEQ-2000 platform.

    Electrophoresis:

    Article Title: Super-pangenome analysis of 3562 human and animal papillomavirus isolates illuminates their genome and pathogenicity evolution
    Article Snippet: Final extension was set at 72 D for 5 min. For PCR product clean-up, we used the 1× VAHTS DNA Clean Beads (Cat: N411, Vazyme, China). .. The obtained HPV16 amplicons, containing three purified PCR amplicon fragments which were examined by electrophoresis and further quantified by Qubit 4 Fluorometer (Thermo Fisher, USA). .. For each sample, we mixed the three PCR amplicon fragments with a 1:1:1 mass ratio and sent for sequencing by BGI Genomics (China) using the BGI MGISEQ-2000 platform.

    Real-time Polymerase Chain Reaction:

    Article Title: Novel compound heterozygous CNGA3 mutation associated with retinal cone dystrophy
    Article Snippet: The NovaSeq 6000 S4 Reagent Kit v1.5 (Illumina, Inc.) was used for sequencing. .. Library concentrations were assessed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories Co., Ltd.) and an Invitrogen Qubit 4 Fluorometer (Thermo Fisher Scientific, Inc.). ..



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